Analysis of the cell wall proteome of Brachypodium distachyon young leaves

Brachypodium distachyon as a model plant towards improved biofuel crops:
Search for secreted proteins involved in polysaccharide synthesis and disassembly.
T Douché, H San Clemente,V Burlat, M Zivy, B Valot, R Pont-Lezica, E Jamet
Proteomics, 13 : 2438-2454, 2013. (Full text)

B. distachyon young leaf
(less than 6 cm in length)

In the following schemes, the strategy used for this particular proteomic study is shown in colored boxes. The overall schemes including grey boxes show different possibilities for proteomics studies as discussed in Jamet et al. (2008) Proteomics 8: 893-908.
- First step consists in protein extraction (destructive vs non destructive methods).
- Second step consists in protein separation and identification (direct analysis vs analysis after protein separation).