BLASTX 2.2.6 [Apr-09-2003]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL3a04.xg.2.1
(369 letters)
Database: /db/trembl-ebi/tmp/swall
1,272,877 sequences; 406,886,216 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sptr|Q8H7P9|Q8H7P9 Putative C-4 sterol methyl oxidase. 59 1e-08
sptr|Q8GT72|Q8GT72 Putative sterol 4-alpha-methyl-oxidase. 58 3e-08
sptr|Q8LAD9|Q8LAD9 Putative C-4 sterol methyl oxidase. 58 3e-08
sptr|Q8L7W5|Q8L7W5 AT4g12110/F16J13_180. 58 3e-08
sptr|Q8LB57|Q8LB57 Putative C-4 sterol methyl oxidase. 58 3e-08
sptrnew|AAP54879|AAP54879 Putative C-4 sterol methyl oxidase. 57 5e-08
sptr|Q9AV39|Q9AV39 Putative C-4 sterol methyl oxidase. 57 5e-08
sptr|O49656|O49656 PREDICTED protein. 56 9e-08
sptr|Q9SZ76|Q9SZ76 Putative C-4 sterol methyl oxidase. 52 2e-06
sptr|Q84SA3|Q84SA3 Putative C-4 sterol methyl oxidase. 44 4e-04
sptr|Q8GTA1|Q8GTA1 Putative sterol 4-alpha-methyl-oxidase. 37 0.073
sptr|Q8W5B3|Q8W5B3 Sterol 4-alpha-methyl-oxidase (Fragment). 33 0.62
sptr|Q8W5A2|Q8W5A2 Sterol 4-alpha-methyl-oxidase (Putative C-4 s... 33 0.62
sptr|Q9ZW22|Q9ZW22 Putative C-4 sterol methyl oxidase. 33 0.62
sptr|Q8VWZ8|Q8VWZ8 Sterol 4-alpha-methyl-oxidase. 33 0.81
sptr|Q8LBE1|Q8LBE1 Putative C-4 sterol methyl oxidase. 33 0.81
sptr|Q9LNW2|Q9LNW2 F22G5.23. 33 0.81
sptr|Q8IIC2|Q8IIC2 Hypothetical protein. 33 1.1
sptrnew|BAC84602|BAC84602 Putative sterol 4-alpha-methyl-oxidase. 30 9.0
>sptr|Q8H7P9|Q8H7P9 Putative C-4 sterol methyl oxidase.
Length = 284
Score = 58.9 bits (141), Expect = 1e-08
Identities = 23/35 (65%), Positives = 32/35 (91%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
G ++SNFSSVFT+CDYIYGT++GY YHK SL++++
Sbjct: 245 GHSQSNFSSVFTFCDYIYGTDRGYRYHKASLSKMR 279
>sptr|Q8GT72|Q8GT72 Putative sterol 4-alpha-methyl-oxidase.
Length = 299
Score = 57.8 bits (138), Expect = 3e-08
Identities = 23/37 (62%), Positives = 32/37 (86%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXK 112
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEK 284
>sptr|Q8LAD9|Q8LAD9 Putative C-4 sterol methyl oxidase.
Length = 299
Score = 57.8 bits (138), Expect = 3e-08
Identities = 23/37 (62%), Positives = 32/37 (86%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXK 112
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEK 284
>sptr|Q8L7W5|Q8L7W5 AT4g12110/F16J13_180.
Length = 298
Score = 57.8 bits (138), Expect = 3e-08
Identities = 25/46 (54%), Positives = 36/46 (78%), Gaps = 3/46 (6%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELK---XKEAEHSG 130
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K +H+G
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNKHNG 293
>sptr|Q8LB57|Q8LB57 Putative C-4 sterol methyl oxidase.
Length = 298
Score = 57.8 bits (138), Expect = 3e-08
Identities = 25/46 (54%), Positives = 36/46 (78%), Gaps = 3/46 (6%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELK---XKEAEHSG 130
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K +H+G
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNKHNG 293
>sptrnew|AAP54879|AAP54879 Putative C-4 sterol methyl oxidase.
Length = 301
Score = 57.0 bits (136), Expect = 5e-08
Identities = 29/57 (50%), Positives = 39/57 (68%)
Frame = +2
Query: 5 KTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXKEAEHSGKED*DNVLSTFYARI 175
+++SNF+SVFTYCDY+YGT+KGY YHK A++K K D N LS YA++
Sbjct: 247 QSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMKALGQTEGEKAD-SNGLS--YAKL 300
>sptr|Q9AV39|Q9AV39 Putative C-4 sterol methyl oxidase.
Length = 301
Score = 57.0 bits (136), Expect = 5e-08
Identities = 29/57 (50%), Positives = 39/57 (68%)
Frame = +2
Query: 5 KTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXKEAEHSGKED*DNVLSTFYARI 175
+++SNF+SVFTYCDY+YGT+KGY YHK A++K K D N LS YA++
Sbjct: 247 QSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMKALGQTEGEKAD-SNGLS--YAKL 300
>sptr|O49656|O49656 PREDICTED protein.
Length = 820
Score = 56.2 bits (134), Expect = 9e-08
Identities = 23/37 (62%), Positives = 31/37 (83%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXK 112
G+++SNF+SVFTYCDYIYGT+KGY + K+ L + K K
Sbjct: 258 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQKKVK 294
Score = 44.3 bits (103), Expect = 4e-04
Identities = 17/24 (70%), Positives = 23/24 (95%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGY 73
G+++SNF+SVFTYCDYIYGT+K +
Sbjct: 463 GQSQSNFASVFTYCDYIYGTDKRF 486
>sptr|Q9SZ76|Q9SZ76 Putative C-4 sterol methyl oxidase.
Length = 303
Score = 51.6 bits (122), Expect = 2e-06
Identities = 25/51 (49%), Positives = 36/51 (70%), Gaps = 8/51 (15%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNK-----GYMYHKRSLAELK---XKEAEHSG 130
G+++SNF+SVFTYCDYIYGT+K GY + K+ L ++K K +H+G
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKMWLYQGYRFQKKLLEQIKESSKKSNKHNG 298
>sptr|Q84SA3|Q84SA3 Putative C-4 sterol methyl oxidase.
Length = 297
Score = 44.3 bits (103), Expect = 4e-04
Identities = 19/33 (57%), Positives = 26/33 (78%), Gaps = 1/33 (3%)
Frame = +2
Query: 5 KTRSNF-SSVFTYCDYIYGTNKGYMYHKRSLAE 100
K++SNF +FTYCDY+YGT+KGY K+ LA+
Sbjct: 247 KSQSNFWHPIFTYCDYLYGTDKGYRNRKKLLAQ 279
>sptr|Q8GTA1|Q8GTA1 Putative sterol 4-alpha-methyl-oxidase.
Length = 269
Score = 36.6 bits (83), Expect = 0.073
Identities = 18/40 (45%), Positives = 23/40 (57%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELKXKEAEHSGKE 136
N+SS F Y D+I+GT+KGY R L LK E K+
Sbjct: 233 NYSSTFVYMDWIFGTDKGY----RKLKALKRDGVEEEAKQ 268
>sptr|Q8W5B3|Q8W5B3 Sterol 4-alpha-methyl-oxidase (Fragment).
Length = 239
Score = 33.5 bits (75), Expect = 0.62
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
N+SS F Y D+I+GT+KGY R L LK
Sbjct: 212 NYSSTFVYMDWIFGTDKGY----RKLKALK 237
>sptr|Q8W5A2|Q8W5A2 Sterol 4-alpha-methyl-oxidase (Putative C-4
sterol methyl oxidase).
Length = 260
Score = 33.5 bits (75), Expect = 0.62
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
N+SS F Y D+I+GT+KGY R L LK
Sbjct: 233 NYSSTFVYMDWIFGTDKGY----RKLKALK 258
>sptr|Q9ZW22|Q9ZW22 Putative C-4 sterol methyl oxidase.
Length = 272
Score = 33.5 bits (75), Expect = 0.62
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
N+SS F Y D+I+GT+KGY R L LK
Sbjct: 245 NYSSTFVYMDWIFGTDKGY----RKLKALK 270
>sptr|Q8VWZ8|Q8VWZ8 Sterol 4-alpha-methyl-oxidase.
Length = 266
Score = 33.1 bits (74), Expect = 0.81
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGY 73
N+SS F Y D+I+GT+KGY
Sbjct: 233 NYSSTFVYMDWIFGTDKGY 251
>sptr|Q8LBE1|Q8LBE1 Putative C-4 sterol methyl oxidase.
Length = 266
Score = 33.1 bits (74), Expect = 0.81
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGY 73
N+SS F Y D+I+GT+KGY
Sbjct: 233 NYSSTFVYMDWIFGTDKGY 251
>sptr|Q9LNW2|Q9LNW2 F22G5.23.
Length = 261
Score = 33.1 bits (74), Expect = 0.81
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGY 73
N+SS F Y D+I+GT+KGY
Sbjct: 228 NYSSTFVYMDWIFGTDKGY 246
>sptr|Q8IIC2|Q8IIC2 Hypothetical protein.
Length = 1653
Score = 32.7 bits (73), Expect = 1.1
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +2
Query: 41 CDYIYGTNKGYMYHKRSLAELKXKEAEHSGKED*DNVLSTFYARISWILHKASIIYSSCS 220
C + Y T+ + L E K + E+ K+ D ++ R +W LH+A +SSC
Sbjct: 1045 CPFPYNTSSEKQ--RNILVESKNLKYENINKKTKDEEYCDYFKRYNWTLHRAE-TFSSCK 1101
Query: 221 LRSV 232
+R +
Sbjct: 1102 IRGI 1105
>sptrnew|BAC84602|BAC84602 Putative sterol 4-alpha-methyl-oxidase.
Length = 268
Score = 29.6 bits (65), Expect = 9.0
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELKXKE 115
N+SS F Y D+++GT+K Y R L+ KE
Sbjct: 235 NYSSTFIYMDWLFGTDKDY----RKTKALEEKE 263
Database: /db/trembl-ebi/tmp/swall
Posted date: Sep 26, 2003 8:29 PM
Number of letters in database: 406,886,216
Number of sequences in database: 1,272,877
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 282,090,759
Number of Sequences: 1272877
Number of extensions: 5174483
Number of successful extensions: 11027
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 10831
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 11027
length of database: 406,886,216
effective HSP length: 98
effective length of database: 282,144,270
effective search space used: 6771462480
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)