TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL3a04.xg.2.1
(369 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA618261.1|CA618261 wl1n.pk0031.e9 wl1n Triticum aestivu... 74 4e-024
>gb|CA618261.1|CA618261 wl1n.pk0031.e9 wl1n Triticum aestivum cDNA clone wl1n.pk0031.e9 5'
end, mRNA sequence
Length = 534
Score = 74.4 bits (156), Expect(5) = 1e-022
Identities = 28/31 (90%), Positives = 29/31 (93%)
Frame = -2 / -1
Query: 95 LGFSCGTCSLCLSRIYNHST*TRN*NCSWSS 3
+GFSCGTCSLCLS IYNH T*TRN*NCSWSS
Sbjct: 342 VGFSCGTCSLCLSHIYNHRT*TRN*NCSWSS 250
Score = 70.7 bits (148), Expect(5) = 4e-024
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = +1 / +2
Query: 4 EDQEQFQFRVHVL*LYIRDKQRLHVPQEKPS 96
EDQEQFQFRVHVL*LYI DKQRLHVPQEKP+
Sbjct: 251 EDQEQFQFRVHVL*LYIWDKQRLHVPQEKPT 343
Score = 36.8 bits (74), Expect(5) = 4e-024
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = +2 / +2
Query: 98 ELKXKEAEHSGKED*D 145
ELK KEAEHSGKED*D
Sbjct: 344 ELKTKEAEHSGKED*D 391
Score = 35.0 bits (70), Expect(5) = 1e-022
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = -2 / -2
Query: 143 LSLLSRCVRPPXXSAR 96
LSLLSRCVRPP SAR
Sbjct: 389 LSLLSRCVRPPSSSAR 342
Score = 27.2 bits (53), Expect(5) = 4e-024
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +3 / +2
Query: 126 AGKKTEITYSQHFMHE*V 179
+GK+ YSQHFMHE*+
Sbjct: 371 SGKED*DYYSQHFMHE*L 424
Score = 23.5 bits (45), Expect(5) = 1e-022
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -2 / -3
Query: 176 LFVHKMLRV 150
LFVHKMLRV
Sbjct: 421 LFVHKMLRV 395
Score = 20.8 bits (39), Expect(5) = 4e-024
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = +1 / +1
Query: 214 LLAPFCSV 237
LLAPFCS+
Sbjct: 460 LLAPFCSL 483
Score = 20.8 bits (39), Expect(5) = 4e-024
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = +2 / +3
Query: 176 SWILHKASI 202
+WILHK S+
Sbjct: 420 NWILHKLSV 446
Score = 19.4 bits (36), Expect(5) = 1e-022
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -2 / -2
Query: 233 EQNGASN 213
EQNGASN
Sbjct: 479 EQNGASN 459
Score = 18.5 bits (34), Expect(5) = 1e-022
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -2 / -3
Query: 209 CI*WTL 192
CI*WTL
Sbjct: 457 CI*WTL 440
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 360,409,806
Number of Sequences: 636343
Number of extensions: 4860939
Number of successful extensions: 240126
Number of sequences better than 1.0e-020: 1
length of database: 122,413,413
effective HSP length: 51
effective length of database: 89,959,920
effective search space used: 6387154320
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)