TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3995470.2.1
(744 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CL173039.1|CL173039 104_376_10890604_116_31795_124 Sorgh... 108 2e-036
gb|CW099509.1|CW099509 104_466_11003588_148_34377_072 Sorgh... 108 1e-035
gb|CW453023.1|CW453023 fsbb001f193n14f0 Sorghum methylation... 108 3e-034
gb|CL162553.1|CL162553 104_355_10806495_116_31833_015 Sorgh... 102 2e-030
gb|BE356845.1|BE356845 DG1_145_G02.b1_A002 Dark Grown 1 (DG... 90 2e-025
gb|BE356942.1|BE356942 DG1_145_G02.g1_A002 Dark Grown 1 (DG... 90 2e-025
gb|CW465791.1|CW465791 fsbb001f216l12k0 Sorghum methylation... 90 8e-025
gb|CW274970.1|CW274970 104_748_11404711_116_35378_030 Sorgh... 90 3e-022
gb|CL192386.1|CL192386 104_415_10940198_116_32263_061 Sorgh... 60 7e-022
>gb|CL173039.1|CL173039 104_376_10890604_116_31795_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10890604, DNA
sequence
Length = 714
Score = 108 bits (231), Expect(3) = 2e-036
Identities = 48/61 (78%), Positives = 51/61 (83%)
Frame = +2 / +3
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR*LQVQVPAMPLAAP 376
KLLKETCAY+KSLHREVDDLSERLSGLM+TMDNDSPQAEIIRSLLR* PA P
Sbjct: 411 KLLKETCAYIKSLHREVDDLSERLSGLMSTMDNDSPQAEIIRSLLR*PGHATPARAAGGP 590
Query: 377 V 379
+
Sbjct: 591 L 593
Score = 93.6 bits (198), Expect(3) = 2e-029
Identities = 38/40 (95%), Positives = 38/40 (95%)
Frame = -3 / -2
Query: 337 SPEEAPDDLGLGAVVVHGRHEPRQPLREVVHLPVQALDVG 218
SPEEAPDDLGL AVVVHGRHEPRQP REVVHLPVQALDVG
Sbjct: 551 SPEEAPDDLGLWAVVVHGRHEPRQPFREVVHLPVQALDVG 432
Score = 59.3 bits (123), Expect(3) = 2e-036
Identities = 26/32 (81%), Positives = 26/32 (81%)
Frame = +2 / +3
Query: 62 PAGDMSSGGRRGRISDDEINELISKLQALLPE 157
P S GRRGRISDDEINELISKLQALLPE
Sbjct: 39 PLAGEMSSGRRGRISDDEINELISKLQALLPE 134
Score = 51.0 bits (105), Expect(3) = 2e-029
Identities = 22/34 (64%), Positives = 25/34 (73%)
Frame = -3 / -2
Query: 160 GFREESLELGDQLVDLVVADPATSAARRHISGRR 59
G RE+ L+LGD+LVDLVVADPAT A R RR
Sbjct: 137 GLREQRLQLGDELVDLVVADPATPARRHFSGERR 36
Score = 26.7 bits (52), Expect(3) = 2e-036
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = +3 / +2
Query: 513 CYGDDLNCVPLFL 551
CY DDLN V LFL
Sbjct: 674 CYDDDLNYVTLFL 712
Score = 26.7 bits (52), Expect(3) = 2e-029
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1 / -1
Query: 435 LRQLARWQAAEEGGRARRTTGAASG 361
L+QLARW+A RAR G +G
Sbjct: 618 LQQLARWRAEGHRPRARGWHGRVTG 544
>gb|CW099509.1|CW099509 104_466_11003588_148_34377_072 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11003588, DNA
sequence
Length = 713
Score = 108 bits (231), Expect(5) = 1e-035
Identities = 48/61 (78%), Positives = 51/61 (83%)
Frame = +2 / +2
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR*LQVQVPAMPLAAP 376
KLLKETCAY+KSLHREVDDLSERLSGLM+TMDNDSPQAEIIRSLLR* PA P
Sbjct: 95 KLLKETCAYIKSLHREVDDLSERLSGLMSTMDNDSPQAEIIRSLLR*PGHATPARAAGGP 274
Query: 377 V 379
+
Sbjct: 275 L 277
Score = 93.6 bits (198), Expect(3) = 1e-023
Identities = 38/40 (95%), Positives = 38/40 (95%)
Frame = -3 / -2
Query: 337 SPEEAPDDLGLGAVVVHGRHEPRQPLREVVHLPVQALDVG 218
SPEEAPDDLGL AVVVHGRHEPRQP REVVHLPVQALDVG
Sbjct: 235 SPEEAPDDLGLWAVVVHGRHEPRQPFREVVHLPVQALDVG 116
Score = 36.8 bits (74), Expect(5) = 1e-035
Identities = 14/18 (77%), Positives = 17/18 (94%)
Frame = +3 / +1
Query: 594 CLFPC*ISSRARALAQKK 647
CLFPC I+SRARALA+K+
Sbjct: 427 CLFPCLINSRARALARKR 480
Score = 32.2 bits (64), Expect(5) = 1e-035
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +3 / +1
Query: 510 ICYGDDLNCVPLFLICSXXXXXXXXSGACL 599
+CY DDLN V LFLI S CL
Sbjct: 355 VCYDDDLNYVTLFLISSSSSSDVVCLFPCL 444
Score = 28.1 bits (55), Expect(3) = 1e-023
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -3 / -1
Query: 598 KHAPEXXXXXXXGEQIKNKGTQLRSSP*QINE 503
KH + E+IKNK T LRSS *Q ++
Sbjct: 443 KHGNKHTTSEEEEEEIKNKVT*LRSSS*QTSK 348
Score = 26.7 bits (52), Expect(3) = 1e-023
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1 / -1
Query: 435 LRQLARWQAAEEGGRARRTTGAASG 361
L+QLARW+A RAR G +G
Sbjct: 302 LQQLARWRAEGHRPRARGWHGRVTG 228
Score = 26.3 bits (51), Expect(5) = 1e-035
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1 / +1
Query: 634 LLRRRRPKVLYSIFSCTT 687
L R+RR + SIFSCTT
Sbjct: 466 LARKRRRPRVLSIFSCTT 519
Score = 22.1 bits (42), Expect(5) = 1e-035
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2 / +3
Query: 719 MDFY*PTK 742
MDFY*PTK
Sbjct: 555 MDFY*PTK 578
>gb|CW453023.1|CW453023 fsbb001f193n14f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f193n14, DNA
sequence
Length = 580
Score = 108 bits (231), Expect(4) = 3e-034
Identities = 48/61 (78%), Positives = 51/61 (83%)
Frame = +2 / -2
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR*LQVQVPAMPLAAP 376
KLLKETCAY+KSLHREVDDLSERLSGLM+TMDNDSPQAEIIRSLLR* PA P
Sbjct: 498 KLLKETCAYIKSLHREVDDLSERLSGLMSTMDNDSPQAEIIRSLLR*PGHATPARAAGGP 319
Query: 377 V 379
+
Sbjct: 318 L 316
Score = 93.6 bits (198), Expect(3) = 1e-023
Identities = 38/40 (95%), Positives = 38/40 (95%)
Frame = -3 / +1
Query: 337 SPEEAPDDLGLGAVVVHGRHEPRQPLREVVHLPVQALDVG 218
SPEEAPDDLGL AVVVHGRHEPRQP REVVHLPVQALDVG
Sbjct: 358 SPEEAPDDLGLWAVVVHGRHEPRQPFREVVHLPVQALDVG 477
Score = 36.8 bits (74), Expect(4) = 3e-034
Identities = 14/18 (77%), Positives = 17/18 (94%)
Frame = +3 / -1
Query: 594 CLFPC*ISSRARALAQKK 647
CLFPC I+SRARALA+K+
Sbjct: 166 CLFPCLINSRARALARKR 113
Score = 32.2 bits (64), Expect(4) = 3e-034
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +3 / -1
Query: 510 ICYGDDLNCVPLFLICSXXXXXXXXSGACL 599
+CY DDLN V LFLI S CL
Sbjct: 238 VCYDDDLNYVTLFLISSSSSSDVVCLFPCL 149
Score = 28.1 bits (55), Expect(3) = 1e-023
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -3 / +3
Query: 598 KHAPEXXXXXXXGEQIKNKGTQLRSSP*QINE 503
KH + E+IKNK T LRSS *Q ++
Sbjct: 150 KHGNKHTTSEEEEEEIKNKVT*LRSSS*QTSK 245
Score = 26.7 bits (52), Expect(3) = 1e-023
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1 / +3
Query: 435 LRQLARWQAAEEGGRARRTTGAASG 361
L+QLARW+A RAR G +G
Sbjct: 291 LQQLARWRAEGHRPRARGWHGRVTG 365
Score = 26.3 bits (51), Expect(4) = 3e-034
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1 / -1
Query: 634 LLRRRRPKVLYSIFSCTT 687
L R+RR + SIFSCTT
Sbjct: 127 LARKRRRPRVLSIFSCTT 74
>gb|CL162553.1|CL162553 104_355_10806495_116_31833_015 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10806495, DNA
sequence
Length = 578
Score = 102 bits (218), Expect(4) = 2e-030
Identities = 45/58 (77%), Positives = 48/58 (82%)
Frame = +2 / +3
Query: 206 KETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR*LQVQVPAMPLAAPV 379
KETCAY+KSLHREVDDLSERLSGLM+TMDNDSPQAEIIRSLLR* PA P+
Sbjct: 3 KETCAYIKSLHREVDDLSERLSGLMSTMDNDSPQAEIIRSLLR*PGHATPARAAGGPL 176
Score = 93.6 bits (198), Expect(3) = 1e-023
Identities = 38/40 (95%), Positives = 38/40 (95%)
Frame = -3 / -1
Query: 337 SPEEAPDDLGLGAVVVHGRHEPRQPLREVVHLPVQALDVG 218
SPEEAPDDLGL AVVVHGRHEPRQP REVVHLPVQALDVG
Sbjct: 134 SPEEAPDDLGLWAVVVHGRHEPRQPFREVVHLPVQALDVG 15
Score = 33.6 bits (67), Expect(4) = 2e-030
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +3 / +2
Query: 594 CLFPC*ISSRARALAQ 641
CLFPC I+SRARALA+
Sbjct: 326 CLFPCLINSRARALAR 373
Score = 32.2 bits (64), Expect(4) = 2e-030
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +3 / +2
Query: 510 ICYGDDLNCVPLFLICSXXXXXXXXSGACL 599
+CY DDLN V LFLI S CL
Sbjct: 254 VCYDDDLNYVTLFLISSSSSSDVVCLFPCL 343
Score = 28.1 bits (55), Expect(3) = 1e-023
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -3 / -3
Query: 598 KHAPEXXXXXXXGEQIKNKGTQLRSSP*QINE 503
KH + E+IKNK T LRSS *Q ++
Sbjct: 342 KHGNKHTTSEEEEEEIKNKVT*LRSSS*QTSK 247
Score = 26.7 bits (52), Expect(3) = 1e-023
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1 / -3
Query: 435 LRQLARWQAAEEGGRARRTTGAASG 361
L+QLARW+A RAR G +G
Sbjct: 201 LQQLARWRAEGHRPRARGWHGRVTG 127
Score = 22.1 bits (42), Expect(4) = 2e-030
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2 / +1
Query: 719 MDFY*PTK 742
MDFY*PTK
Sbjct: 454 MDFY*PTK 477
>gb|BE356845.1|BE356845 DG1_145_G02.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 583
Score = 90.4 bits (191), Expect(2) = 2e-025
Identities = 37/46 (80%), Positives = 44/46 (95%)
Frame = +2 / +1
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++SP AEIIRS+LR
Sbjct: 382 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSPGAEIIRSILR 519
Score = 46.4 bits (95), Expect(2) = 2e-025
Identities = 20/25 (80%), Positives = 21/25 (84%)
Frame = +2 / +1
Query: 80 SGGRRGRISDDEINELISKLQALLP 154
S RG IS+DEINELISKLQALLP
Sbjct: 268 SSSSRGNISEDEINELISKLQALLP 342
>gb|BE356942.1|BE356942 DG1_145_G02.g1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 535
Score = 90.4 bits (191), Expect(2) = 2e-025
Identities = 37/46 (80%), Positives = 44/46 (95%)
Frame = +2 / +3
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++SP AEIIRS+LR
Sbjct: 204 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSPGAEIIRSILR 341
Score = 46.4 bits (95), Expect(2) = 2e-025
Identities = 20/25 (80%), Positives = 21/25 (84%)
Frame = +2 / +3
Query: 80 SGGRRGRISDDEINELISKLQALLP 154
S RG IS+DEINELISKLQALLP
Sbjct: 90 SSSSRGNISEDEINELISKLQALLP 164
>gb|CW465791.1|CW465791 fsbb001f216l12k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f216l12, DNA
sequence
Length = 644
Score = 90.4 bits (191), Expect(2) = 8e-025
Identities = 37/46 (80%), Positives = 44/46 (95%)
Frame = +2 / -3
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++SP AEIIRS+LR
Sbjct: 144 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSPGAEIIRSILR 7
Score = 46.4 bits (95), Expect(2) = 8e-025
Identities = 20/25 (80%), Positives = 21/25 (84%)
Frame = +2 / -2
Query: 80 SGGRRGRISDDEINELISKLQALLP 154
S RG IS+DEINELISKLQALLP
Sbjct: 490 SSSSRGNISEDEINELISKLQALLP 416
>gb|CW274970.1|CW274970 104_748_11404711_116_35378_030 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11404711, DNA
sequence
Length = 717
Score = 90.4 bits (191), Expect(2) = 3e-022
Identities = 37/46 (80%), Positives = 44/46 (95%)
Frame = +2 / +2
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++SP AEIIRS+LR
Sbjct: 323 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSPGAEIIRSILR 460
Score = 38.2 bits (77), Expect(2) = 3e-022
Identities = 16/17 (94%), Positives = 17/17 (100%)
Frame = +2 / +1
Query: 104 SDDEINELISKLQALLP 154
S+DEINELISKLQALLP
Sbjct: 1 SEDEINELISKLQALLP 51
>gb|CL192386.1|CL192386 104_415_10940198_116_32263_061 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10940198, DNA
sequence
Length = 609
Score = 60.2 bits (125), Expect(5) = 7e-022
Identities = 28/40 (70%), Positives = 30/40 (75%)
Frame = +2 / -3
Query: 260 ERLSGLMATMDNDSPQAEIIRSLLR*LQVQVPAMPLAAPV 379
ERLSGLM+TMDNDSPQAEIIRSLLR* PA P+
Sbjct: 607 ERLSGLMSTMDNDSPQAEIIRSLLR*PGHATPARAAGGPL 488
Score = 36.8 bits (74), Expect(5) = 7e-022
Identities = 14/18 (77%), Positives = 17/18 (94%)
Frame = +3 / -2
Query: 594 CLFPC*ISSRARALAQKK 647
CLFPC I+SRARALA+K+
Sbjct: 338 CLFPCLINSRARALARKR 285
Score = 32.2 bits (64), Expect(5) = 7e-022
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +3 / -2
Query: 510 ICYGDDLNCVPLFLICSXXXXXXXXSGACL 599
+CY DDLN V LFLI S CL
Sbjct: 410 VCYDDDLNYVTLFLISSSSSSDVVCLFPCL 321
Score = 26.3 bits (51), Expect(5) = 7e-022
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1 / -2
Query: 634 LLRRRRPKVLYSIFSCTT 687
L R+RR + SIFSCTT
Sbjct: 299 LARKRRRPRVLSIFSCTT 246
Score = 22.1 bits (42), Expect(5) = 7e-022
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2 / -1
Query: 719 MDFY*PTK 742
MDFY*PTK
Sbjct: 210 MDFY*PTK 187
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 751,597,545
Number of Sequences: 832831
Number of extensions: 8974833
Number of successful extensions: 126668
Number of sequences better than 1.0e-020: 9
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 22931040626
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)