TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS9f07.yg.2.1
(394 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AJ891040.1| Populus tremula x Populus alba mRNA for alp... 242 8e-064
gb|DT526453.1|DT526453 WS02048.C21_B09 PTxN-IB-N-A-11 Popul... 118 2e-026
gb|CV272375.1|CV272375 WS0157.B21_H14 PTxN-IB-A-6 Populus t... 102 1e-021
gb|BP928380.1|BP928380 BP928380 full-length enriched poplar... 32 2.2
gb|AC149429.1| Populus trichocarpa clone Pop1-109D09, compl... 32 2.2
gb|CN518652.1|CN518652 GQ0104.B3_N14 GQ010 Populus trichoca... 31 3.0
gb|CN520560.1|CN520560 GQ0105.B3_E01 GQ010 Populus trichoca... 31 3.0
gb|CV254523.1|CV254523 WS0241.B21_H10 PTxD-ICC-N-A-14 Popul... 31 4.1
gb|DT489625.1|DT489625 WS02542.B21_L21 PT-MB-N-A-15 Populus... 31 4.1
gb|DT490401.1|DT490401 WS02544.B21_N21 PT-MB-N-A-15 Populus... 31 4.1
gb|CX658495.1|CX658495 PO01035G03 Poplar SC cDNA library Po... 26 5.0
gb|CV254449.1|CV254449 WS0241.B21_E05 PTxD-ICC-N-A-14 Popul... 30 5.6
gb|BI139456.1|BI139456 F131P95Y Populus flower cDNA library... 24 6.5
gb|CX173827.1|CX173827 A02_69-82_02.ab1 leaf inoculated wit... 22 9.4
gb|CX175650.1|CX175650 C12_69-85_06.ab1 leaf inoculated wit... 22 9.9
>emb|AJ891040.1| Populus tremula x Populus alba mRNA for alpha 1,3 fucosyltransferase
(poFUT2 gene), clone INRA 717 1B4
Length = 1569
Score = 242 bits (523), Expect = 8e-064
Identities = 94/131 (71%), Positives = 111/131 (84%)
Frame = -1 / +1
Query: 394 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSSKKGTVYHLF 215
KY+GPSDSFKAL+DMAAVHSSCRLCIH+AT I KEE +P F RPC C+ TVYHL+
Sbjct: 1090 KYEGPSDSFKALVDMAAVHSSCRLCIHLATMIREKEENSPGFKRRPCRCTKDLETVYHLY 1269
Query: 214 IRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRI 35
+RERGRF+ ESI++RSG LT+ ALE+AVL KF+SL HVP+WK ERP SIRGGDD K+YR+
Sbjct: 1270 VRERGRFQMESIFLRSGNLTVNALEAAVLKKFKSLKHVPIWKQERPESIRGGDDFKVYRV 1449
Query: 34 YPVGLTQRQAL 2
YPVG+TQRQAL
Sbjct: 1450 YPVGMTQRQAL 1482
Score = 107 bits (228), Expect = 4e-023
Identities = 62/126 (49%), Positives = 72/126 (57%)
Frame = +3 / -2
Query: 15 CVRPTG*ILYSFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPSVNWPDLI*MLSDL 194
CV PTG L + + F GT +DLN STA S+A +V PDL M S
Sbjct: 1469 CVMPTGYTL*TLKSSPPLMLSGLSCFQMGTCFKDLNFLSTAASRAFTVRLPDLKKMDSI* 1290
Query: 195 NRPLSRINKW*TVPFLLEQLQGRFVNFGVLSSFK*ILVAICIQRRQDE*TAAMSIRALKE 374
N PLS K TV L LQGR +N G+ SSF I+VA +QRR DE TAA+S ALKE
Sbjct: 1289 NLPLSLTYK*YTVSKSLVHLQGRLLNPGLFSSFSRIIVAR*MQRRHDECTAAISTSALKE 1110
Query: 375 SDGPSY 392
SDGPSY
Sbjct: 1109 SDGPSY 1092
Score = 58.8 bits (122), Expect = 2e-008
Identities = 31/89 (34%), Positives = 51/89 (57%)
Frame = -3 / +3
Query: 380 I*FFQSSYRHGSGSFILSSLYTYCYQDSFKGGKDSKIYKSTL*LFQQKGDSLPLVYPRER 201
I*FFQ + R+GS + I+ SL++ CY DS + GK ++ +++L + Q+ D + + RER
Sbjct: 1104 I*FFQGTGRYGSSTLIMPSLHSPCYYDSGERGK*PRV*EASLQMHQRLRDRVSFICKRER 1283
Query: 200 AV*VREHLYEIGPVNTGSLGICSAR*I*V 114
+ H E+ N CSA+ +*V
Sbjct: 1284 KISDGVHFLEVWQSNCKCSRGCSAQEV*V 1370
Score = 43.2 bits (88), Expect(2) = 9e-010
Identities = 26/70 (37%), Positives = 36/70 (51%)
Frame = +2 / -1
Query: 2 QSLTLC*TDWVNSV*FQVIPTSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC*LARSHIN 181
QSL LC V SV F+++ + F+LP ++++ K EHC *+AR N
Sbjct: 1482 QSLPLCHAYRVYSVNFKIVSSPYAFRSFLLPDGHMLQRLKLLEHCSL*SIYS*IARPQEN 1303
Query: 182 AL*LKPPSLS 211
L LK SLS
Sbjct: 1302 GLHLKSSSLS 1273
Score = 38.2 bits (77), Expect(2) = 9e-010
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = +2 / -1
Query: 245 GTATRSICKFWSPFLL*MNLGSNMYTKTTR*MNRCHVDKSFERIRWTII 391
G + + K W+ FL N S + KT *+ CH+ + ERIRW ++
Sbjct: 1239 GASAGTPLKPWAIFLFLPNHSSKVNAKTA**VYCCHIYQCLERIRWALV 1093
Score = 34.1 bits (68), Expect = 0.44
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = -2 / +2
Query: 381 HLILSKLLSTWQRFIHLVVFVYILLPRFI*RRKGLQNLQ 265
HLILS+ WQ++ H VF + LL F +RK Q L+
Sbjct: 1103 HLILSRHW*IWQQYTHHAVFAFTLLL*FGRKRKIAQGLR 1219
>gb|DT526453.1|DT526453 WS02048.C21_B09 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02048_B09 3', mRNA sequence
Length = 677
Score = 118 bits (252), Expect = 2e-026
Identities = 46/62 (74%), Positives = 55/62 (88%)
Frame = -1 / -3
Query: 187 ESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQ 8
ESI++RSG LT+ ALE+AVL KF+SL HVP+WK ERP SIRGGDD K+YR+YPVG+TQRQ
Sbjct: 669 ESIFLRSGNLTVKALEAAVLKKFKSLKHVPIWKQERPESIRGGDDFKVYRVYPVGMTQRQ 490
Query: 7 AL 2
AL
Sbjct: 489 AL 484
Score = 40.9 bits (83), Expect = 0.004
Identities = 21/53 (39%), Positives = 26/53 (49%)
Frame = +3 / +2
Query: 15 CVRPTG*ILYSFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPSVNWPDL 173
CV PTG L + + F GT +DLN STA S+A +V PDL
Sbjct: 497 CVMPTGYTL*TLKSSPPLMLSGLSCFQMGTCFKDLNFLSTAASRAFTVRLPDL 655
Score = 36.3 bits (73), Expect = 0.090
Identities = 21/62 (33%), Positives = 31/62 (50%)
Frame = +2 / +1
Query: 2 QSLTLC*TDWVNSV*FQVIPTSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC*LARSHIN 181
QSL LC V SV F+++ + F+LP ++++ K EHC *+AR N
Sbjct: 484 QSLPLCHAYRVYSVNFKIVSSPYAFRSFLLPDGHMLQRLKLLEHCSL*SLYS*IARPQEN 663
Query: 182 AL 187
L
Sbjct: 664 GL 669
>gb|CV272375.1|CV272375 WS0157.B21_H14 PTxN-IB-A-6 Populus trichocarpa x Populus nigra cDNA
clone WS0157_H14 3', mRNA sequence
Length = 688
Score = 102 bits (217), Expect = 1e-021
Identities = 39/52 (75%), Positives = 46/52 (88%)
Frame = -1 / -3
Query: 157 TLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
T+ ALE+AVL KF+SL HVP+WK ERP SIRGGDD K+YR+YPVG+TQRQAL
Sbjct: 686 TVNALEAAVLKKFKSLKHVPIWKQERPESIRGGDDFKVYRVYPVGMTQRQAL 531
Score = 34.1 bits (68), Expect = 0.44
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = +3 / +1
Query: 15 CVRPTG*ILYSFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPSV 158
CV PTG L + + F GT +DLN STA S+A +V
Sbjct: 544 CVMPTGYTL*TLKSSPPLMLSGLSCFQMGTCFKDLNFLSTAASRAFTV 687
Score = 29.9 bits (59), Expect = 7.7
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2 / +3
Query: 2 QSLTLC*TDWVNSV*FQVIPTSNTRWPFILPYRNVVEGPKFTEHC 136
QSL LC V V F+++ + F+LP ++++ K EHC
Sbjct: 531 QSLPLCHAYRVYPVNFKIVSSPYAFRSFLLPDGHMLQRLKLLEHC 665
>gb|BP928380.1|BP928380 BP928380 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-082_K01.f 5', mRNA sequence
Length = 516
Score = 31.8 bits (63), Expect = 2.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +2 / +3
Query: 62 TSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC 157
T NT+ PF+ P + G K E QG QC
Sbjct: 24 TKNTKTPFLKPEKGPNFGAKMREILHIQGGQC 119
>gb|AC149429.1| Populus trichocarpa clone Pop1-109D09, complete sequence
Length = 94633
Score = 31.8 bits (63), Expect = 2.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +2 / -3
Query: 62 TSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC 157
T NT+ PF+ P + G K E QG QC
Sbjct: 26891 TKNTKTPFLKPEKGPNFGAKMREILHIQGGQC 26796
>gb|CN518652.1|CN518652 GQ0104.B3_N14 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0104_N14 5', mRNA sequence
Length = 772
Score = 31.3 bits (62), Expect = 3.0
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = +2 / -2
Query: 200 PSLSDKQVVNCPLFAGTATRSICKFWSP 283
PSL Q NCP F G A SIC + P
Sbjct: 513 PSLHVLQTDNCPSFNGVALFSICPWLRP 430
>gb|CN520560.1|CN520560 GQ0105.B3_E01 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0105_E01 5', mRNA sequence
Length = 762
Score = 31.3 bits (62), Expect = 3.0
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = +2 / -2
Query: 200 PSLSDKQVVNCPLFAGTATRSICKFWSP 283
PSL Q NCP F G A SIC + P
Sbjct: 197 PSLHVLQTDNCPSFNGVALFSICPWLRP 114
>gb|CV254523.1|CV254523 WS0241.B21_H10 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0241_H10 3', mRNA sequence
Length = 892
Score = 30.9 bits (61), Expect = 4.1
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1 / -1
Query: 265 NRPCSCSSKKGTVYHLFIRE 206
N PC C+ + T HLF RE
Sbjct: 382 NEPCECTKSRTTSQHLFCRE 323
>gb|DT489625.1|DT489625 WS02542.B21_L21 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02542_L21 3', mRNA sequence
Length = 564
Score = 30.9 bits (61), Expect = 4.1
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = -1 / +1
Query: 289 EERTPKFTNRPCSCSSKKGTVYHLFIRERGRFKSESIYMRS 167
EER + ++P S G ++HL I R K+ +I++ S
Sbjct: 28 EERKTIYNSQPV*LHSSIGPIFHLKIHTRNNAKNHTIHLNS 150
>gb|DT490401.1|DT490401 WS02544.B21_N21 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02544_N21 3', mRNA sequence
Length = 691
Score = 30.9 bits (61), Expect = 4.1
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -1 / +3
Query: 346 AVHSSCRLCIHIAT 305
AVH+SCRL +HIAT
Sbjct: 567 AVHASCRLLLHIAT 608
>gb|CX658495.1|CX658495 PO01035G03 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO01035G03 5', mRNA sequence
Length = 648
Score = 26.3 bits (51), Expect(2) = 5.0
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -1 / +2
Query: 112 LNHVPVWKDERPPSIRGGD 56
+NH WK+ P S RGGD
Sbjct: 287 VNHSIFWKNLSPVSERGGD 343
Score = 22.1 bits (42), Expect(2) = 5.0
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -1 / +2
Query: 151 GALESAVLGKFRSLNH 104
GALE A+ G+ L+H
Sbjct: 107 GALEPAISGEIMQLHH 154
>gb|CV254449.1|CV254449 WS0241.B21_E05 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0241_E05 3', mRNA sequence
Length = 550
Score = 30.4 bits (60), Expect = 5.6
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -2 / -1
Query: 153 WEPWNLQCSVNLGPSTTFLYGRMKGHRV 70
W+PW+L S+ L P FL + G V
Sbjct: 97 WQPWSLAVSLGLSPRWIFLLSALCGKGV 14
>gb|BI139456.1|BI139456 F131P95Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA
sequence
Length = 514
Score = 24.4 bits (47), Expect(2) = 6.5
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -1 / +3
Query: 112 LNHVPVWKDERPPSIRGGD 56
+NH WK+ P S RGG+
Sbjct: 294 VNHSIFWKNLSPVSERGGE 350
Score = 22.1 bits (42), Expect(2) = 6.5
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -1 / +3
Query: 151 GALESAVLGKFRSLNH 104
GALE A+ G+ L+H
Sbjct: 114 GALEPAISGEIMQLHH 161
>gb|CX173827.1|CX173827 A02_69-82_02.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 583
Score = 21.7 bits (41), Expect(3) = 9.4
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = -1 / -3
Query: 346 AVHSSCRLC 320
+VH SCR+C
Sbjct: 548 SVHQSCRVC 522
Score = 21.2 bits (40), Expect(3) = 9.4
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = -2 / -3
Query: 132 CSVNLGPSTTF 100
C++NL STTF
Sbjct: 197 CNINLNISTTF 165
Score = 18.0 bits (33), Expect(3) = 9.4
Identities = 4/13 (30%), Positives = 8/13 (61%)
Frame = -1 / -2
Query: 262 RPCSCSSKKGTVY 224
R C CS ++ ++
Sbjct: 477 RKCPCSQRQANIH 439
>gb|CX175650.1|CX175650 C12_69-85_06.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 599
Score = 21.7 bits (41), Expect(3) = 9.9
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = -1 / -1
Query: 346 AVHSSCRLC 320
+VH SCR+C
Sbjct: 458 SVHQSCRVC 432
Score = 21.2 bits (40), Expect(3) = 9.9
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = -2 / -1
Query: 132 CSVNLGPSTTF 100
C++NL STTF
Sbjct: 107 CNINLNISTTF 75
Score = 18.0 bits (33), Expect(3) = 9.9
Identities = 4/13 (30%), Positives = 8/13 (61%)
Frame = -1 / -3
Query: 262 RPCSCSSKKGTVY 224
R C CS ++ ++
Sbjct: 387 RKCPCSQRQANIH 349
Database: Populus_nucl_with_EST.fasta
Posted date: May 2, 2006 3:31 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 192,223,475
Number of Sequences: 369679
Number of extensions: 2344735
Number of successful extensions: 86529
Number of sequences better than 10.0: 17
length of database: 67,802,888
effective HSP length: 50
effective length of database: 49,318,938
effective search space used: 3945515040
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)